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Chrysin inhibits cell invasion by inhibition of Recepteur d'origine Nantais via suppressing early growth response-1 and NF-κB transcription factor activities in gastric cancer cells.
- Source :
-
International journal of oncology [Int J Oncol] 2015 Apr; Vol. 46 (4), pp. 1835-43. Date of Electronic Publication: 2015 Jan 23. - Publication Year :
- 2015
-
Abstract
- Cell invasion is one of crucial reasons for cancer metastasis and malignancy. Recepteur d'origine Nantais (RON) has been reported to play an important role in the cancer cell invasion process. High accumulation and activation of RON has been implicated in gastric adenocarcinoma AGS cells. Chrysin is a naturally occurring phytochemical, a type of flavonoid, which has been reported to suppress tumor metastasis. However, the effects of chrysin on RON expression in gastric cancer are not well studied. In the present study, we examined whether chrysin affects RON expression in gastric cancer, and if so, its underlying mechanism. We examined the effect of chrysin on RON expression and activity, via RT-PCR, promoter study, and western blotting in human gastric cancer AGS cells. Chrysin significantly inhibited endogenous and inducible RON expression in a dose-dependent manner. After demonstrating that Egr-1 and NF-κB are the critically required transcription factors for RON expression, we discovered that chrysin suppressed Egr-1 and NF-κB transcription factor activities. Additionally, the phorbol-12-myristate-13-acetate- (PMA) induced cell invasion was partially abrogated by chrysin and an RON antibody. Our results suggest that chrysin has anticancer effects at least by suppressing RON expression through blocking Egr-1 and NF-κB in gastric cancer AGS cells.
- Subjects :
- Cell Line, Tumor
Cell Movement drug effects
Dose-Response Relationship, Drug
Early Growth Response Protein 1 metabolism
Humans
NF-kappa B genetics
Promoter Regions, Genetic drug effects
Receptor Protein-Tyrosine Kinases metabolism
Stomach Neoplasms genetics
Stomach Neoplasms metabolism
Antineoplastic Agents pharmacology
Early Growth Response Protein 1 genetics
Flavonoids pharmacology
NF-kappa B metabolism
Receptor Protein-Tyrosine Kinases genetics
Stomach Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 46
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 25625479
- Full Text :
- https://doi.org/10.3892/ijo.2015.2847