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Detection and genetic characterization of β-lactamases in Prevotella intermedia and Prevotella nigrescens isolated from oral cavity infections and peritonsillar abscesses.
- Source :
-
Anaerobe [Anaerobe] 2015 Jun; Vol. 33, pp. 8-13. Date of Electronic Publication: 2015 Jan 23. - Publication Year :
- 2015
-
Abstract
- A prospective analysis on β-lactam resistance mechanisms and β-lactamase prevalence was conducted on Prevotella intermedia and Prevotella nigrescens recovered from patients with chronic periodontitis and peritonsillar abscesses. Both phenotypic and genotypic methods were performed to characterize the β-lactamases, their coding genes and their genetic contexts. Overall, β-lactamase production was observed in 64% (16/25) P. intermedia and 23.8% (5/21) P. nigrescens (p < 0.01). Besides higher β-lactamase production rates were observed in P. intermedia (8/16) than in P. nigrescens (2/16) recovered from chronic periodontitis, almost all isolates from peritonsillar abscesses were producers (8/9 and 3/3, respectively). cfxA, but not cepA and cblA, was detected in those isolates, which were previously categorized as β-lactamase producers. CfxA producing isolates displayed higher β-lactam MICs than non-producers in both species. The most frequent allele was cfxA2, followed by cfxA3 and a new allelic variant named cfxA6. The analysis of the downstream flanking region in the three cfxA variants revealed the association with mobA of Tn4555, suggesting their localization in a mobilizable element. β-lactam resistance and cfxA carriage prevalence seems to be not only related to the bacterial species but also to the infection site.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents pharmacology
Bacteroidaceae Infections epidemiology
Female
Gene Order
Genotype
Humans
Male
Microbial Sensitivity Tests
Peritonsillar Abscess epidemiology
Prevalence
Prevotella intermedia drug effects
Prevotella intermedia isolation & purification
Prevotella nigrescens drug effects
Prevotella nigrescens isolation & purification
Prospective Studies
beta-Lactam Resistance
Bacteroidaceae Infections microbiology
Peritonsillar Abscess microbiology
Prevotella intermedia genetics
Prevotella nigrescens genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8274
- Volume :
- 33
- Database :
- MEDLINE
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 25623818
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2015.01.007