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Prevalence of virulence determinants and antimicrobial resistance among commensal Escherichia coli derived from dairy and beef cattle.
Prevalence of virulence determinants and antimicrobial resistance among commensal Escherichia coli derived from dairy and beef cattle.
- Source :
-
International journal of environmental research and public health [Int J Environ Res Public Health] 2015 Jan 19; Vol. 12 (1), pp. 970-85. Date of Electronic Publication: 2015 Jan 19. - Publication Year :
- 2015
-
Abstract
- Cattle is a reservoir of potentially pathogenic E. coli, bacteria that can represent a significant threat to public health, hence it is crucial to monitor the prevalence of the genetic determinants of virulence and antimicrobial resistance among the E. coli population. The aim of this study was the analysis of the phylogenetic structure, distribution of virulence factors (VFs) and prevalence of antimicrobial resistance among E. coli isolated from two groups of healthy cattle: 50 cows housed in the conventional barn (147 isolates) and 42 cows living on the ecological pasture (118 isolates). The phylogenetic analysis, identification of VFs and antimicrobial resistance genes were based on either multiplex or simplex PCR. The antimicrobial susceptibilities of E. coli were examined using the broth microdilution method. Two statistical approaches were used to analyse the results obtained for two groups of cattle. The relations between the dependent (VFs profiles, antibiotics) and the independent variables were described using the two models. The mixed logit model was used to characterise the prevalence of the analysed factors in the sets of isolates. The univariate logistic regression model was used to characterise the prevalence of these factors in particular animals. Given each model, the odds ratio (OR) and the 95% confidence interval for the population were estimated. The phylogroup B1 was predominant among isolates from beef cattle, while the phylogroups A, B1 and D occurred with equal frequency among isolates from dairy cattle. The frequency of VFs-positive isolates was significantly higher among isolates from beef cattle. E. coli from dairy cattle revealed significantly higher resistance to antibiotics. Some of the tested resistance genes were present among isolates from dairy cattle. Our study showed that the habitat and diet may affect the genetic diversity of commensal E. coli in the cattle. The results suggest that the ecological pasture habitat is related to the increased spreading rate of the VFs, while the barn habitat is characterised by the higher levels of antimicrobial resistance among E. coli.
- Subjects :
- Animals
Cattle
Cattle Diseases microbiology
Escherichia coli physiology
Escherichia coli Infections epidemiology
Escherichia coli Infections microbiology
Female
Male
Phylogeny
Poland epidemiology
Prevalence
Virulence Factors
Anti-Infective Agents pharmacology
Cattle Diseases epidemiology
Drug Resistance, Bacterial
Escherichia coli drug effects
Escherichia coli pathogenicity
Escherichia coli Infections veterinary
Organic Agriculture
Subjects
Details
- Language :
- English
- ISSN :
- 1660-4601
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of environmental research and public health
- Publication Type :
- Academic Journal
- Accession number :
- 25607605
- Full Text :
- https://doi.org/10.3390/ijerph120100970