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Planarian myosin essential light chain is involved in the formation of brain lateral branches during regeneration.
- Source :
-
Molecular genetics and genomics : MGG [Mol Genet Genomics] 2015 Aug; Vol. 290 (4), pp. 1277-85. Date of Electronic Publication: 2015 Jan 14. - Publication Year :
- 2015
-
Abstract
- The myosin essential light chain (ELC) is a structure component of the actomyosin cross-bridge, however, the functions in the central nervous system (CNS) development and regeneration remain poorly understood. Planarian Dugesia japonica has revealed fundamental mechanisms and unique aspects of neuroscience and neuroregeneration. In this study, the cDNA DjElc, encoding a planarian essential light chain of myosin, was identified from the planarian Dugesia japonica cDNA library. It encodes a deduced protein with highly conserved functionally domains EF-Hand and Ca(2+) binding sites that shares significant similarity with other members of ELC. Whole mount in situ hybridization studies show that DjElc expressed in CNS during embryonic development and regeneration of adult planarians. Loss of function of DjElc by RNA interference during planarian regeneration inhibits brain lateral branches regeneration completely. In conclusion, these results demonstrated that DjElc is required for maintenance of neurons and neurite outgrowth, particularly for involving the brain later branch regeneration.
- Subjects :
- Animals
Brain embryology
Brain physiology
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Library
In Situ Hybridization
Microscopy, Confocal
Molecular Sequence Data
Myosin Light Chains classification
Neurites metabolism
Neurites physiology
Neurons metabolism
Neurons physiology
Phylogeny
Planarians embryology
Planarians physiology
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Brain metabolism
Helminth Proteins genetics
Myosin Light Chains genetics
Planarians genetics
Regeneration genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1617-4623
- Volume :
- 290
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular genetics and genomics : MGG
- Publication Type :
- Academic Journal
- Accession number :
- 25585662
- Full Text :
- https://doi.org/10.1007/s00438-015-0990-1