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Evidence that vascular actions of PHI are mediated by a VIP-preferring receptor.
- Source :
-
Peptides [Peptides] 1989 Sep-Oct; Vol. 10 (5), pp. 993-1001. - Publication Year :
- 1989
-
Abstract
- Vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) are homologous neuropeptides which share vasodilatory properties. This paper addresses the question of whether PHI exerts its vascular action via a receptor distinct from that for VIP. Radioligand binding experiments were done using [Tyr(125I)10]VIP, [Tyr(125I)22]porcine PHI, [Tyr(125I)10]rat PHI and arterial preparations from rat, bovine and porcine species. The radioiodination of rat PHI by the lactoperoxidase-glucose oxidase method and analysis of the structure of the major radiolabeled derivatives were described. All the receptor binding experiments identified a VIP-preferring receptor irrespective of which radioligand or arterial preparation was utilized. VIP and PHI peptides demonstrated cross-desensitization in studies of relaxation of porcine coronary arterial strips in vitro. The present results favor the conclusion that the vascular actions of the PHI peptides are best explained by binding to a VIP-preferring receptor.
- Subjects :
- Amino Acid Sequence
Animals
Binding, Competitive physiology
Cattle
Coronary Vessels physiology
Humans
In Vitro Techniques
Mesenteric Arteries metabolism
Molecular Sequence Data
Peptide PHI metabolism
Radioligand Assay
Rats
Receptors, Gastrointestinal Hormone metabolism
Receptors, Vasoactive Intestinal Peptide
Swine
Peptide PHI physiology
Receptors, Gastrointestinal Hormone physiology
Vasoactive Intestinal Peptide metabolism
Vasodilation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0196-9781
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Peptides
- Publication Type :
- Academic Journal
- Accession number :
- 2558369
- Full Text :
- https://doi.org/10.1016/0196-9781(89)90181-2