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Anti-obesity effect of total phenylpropanoid glycosides from Ligustrum robustum Blume in fatty diet-fed mice via up-regulating leptin.
- Source :
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Journal of ethnopharmacology [J Ethnopharmacol] 2015 Jul 01; Vol. 169, pp. 459-65. Date of Electronic Publication: 2015 Jan 07. - Publication Year :
- 2015
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Abstract
- Ethnopharmacological Relevance: In Chinese folk medicine, the leaves of Ligustrum robustum Blume (LR) were commonly used in the treatment of obesity and hyperlipidemia. This study aimed to evaluate the anti-obesity effect and mechanisms of total phenylpropanoid glycosides from Ligustrum robustum Blume (LRTPG) in fatty diet-fed C57BL/6J mice.<br />Materials and Methods: C57BL/6J mice were divided randomly into 6 groups, i.e., control, model, positive (Orlistat 0.12g/kg), and LRTPG at three dosages (0.3, 0.6 or 1.2g/kg), respectively. Control mice were fed with standard diet; the others were fed with fatty diet. After 4 weeks׳ modeling, therapy mice were intragastrically administrated with positive drug or LRTPG for 5 weeks, respectively. Pharmacodynamic effects including body weight, fat weight, Lee׳s index, serum lipid levels, morphological changes and adipocyte area ratio were evaluated. The mechanisms were explored as the factors related to lipids metabolism in gene expressions by real-time PCR, and assured as the protein level of differential gene by Western blotting.<br />Results: The anti-obesity effects of LRTPG in all treated mice were shown as decreased body weight, fat mass, Lee׳s index, total cholesterol (TC) level, and adipocyte area. The mechanisms were demonstrated as elevated mRNA and protein levels of adipose leptin, and consequently decreasing mRNA of adipose acyl coenzyme A: diacylglycerol acyltransferase (DGAT) with increasing mRNA of hepatic cholesterol 7α-hydroxylase (CYP7A1), which led to inhibition of triglyceride (TG) synthesis and promotion of cholesterol catabolism.<br />Conclusions: The anti-obesity effect of LRTPG in fatty diet-fed mice was related to the up-regulation of leptin, which may provide scientific evidence supporting the traditional usage of LR on obesity in China.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Acyl Coenzyme A biosynthesis
Adipocytes drug effects
Adipose Tissue drug effects
Adipose Tissue enzymology
Animals
Anti-Obesity Agents isolation & purification
Anti-Obesity Agents pharmacology
Body Weight
Cholesterol 7-alpha-Hydroxylase biosynthesis
Diacylglycerol O-Acyltransferase biosynthesis
Dietary Fats administration & dosage
Dose-Response Relationship, Drug
Glycosides isolation & purification
Glycosides pharmacology
Lipid Metabolism drug effects
Male
Mice
Obesity drug therapy
Plant Extracts chemistry
Plant Leaves chemistry
Up-Regulation
Anti-Obesity Agents therapeutic use
Glycosides therapeutic use
Leptin biosynthesis
Ligustrum chemistry
Phytotherapy methods
Plant Extracts therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 169
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 25576894
- Full Text :
- https://doi.org/10.1016/j.jep.2014.12.066