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How cryo-EM is revolutionizing structural biology.
- Source :
-
Trends in biochemical sciences [Trends Biochem Sci] 2015 Jan; Vol. 40 (1), pp. 49-57. Date of Electronic Publication: 2014 Nov 07. - Publication Year :
- 2015
-
Abstract
- For many years, structure determination of biological macromolecules by cryo-electron microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent advances in electron detection and image processing, the resolution by cryo-EM is now beginning to rival X-ray crystallography. A new generation of electron detectors record images with unprecedented quality, while new image-processing tools correct for sample movements and classify images according to different structural states. Combined, these advances yield density maps with sufficient detail to deduce the atomic structure for a range of specimens. Here, we review the recent advances and illustrate the exciting new opportunities that they offer to structural biology research.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 0968-0004
- Volume :
- 40
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Trends in biochemical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 25544475
- Full Text :
- https://doi.org/10.1016/j.tibs.2014.10.005