Quantitative analysis of CDKN2A methylation, mRNA, and p16(INK4a) protein expression in children and adolescents with Burkitt lymphoma: biological and clinical implications.

Authors :: Robaina MC
Faccion RS
Arruda VO
de Rezende LM
Vasconcelos GM
Apa AG
Bacchi CE
Klumb CE
Source :: Leukemia research [Leuk Res] 2015 Feb; Vol. 39 (2), pp. 248-56. Date of Electronic Publication: 2014 Dec 09.
Publication Year :: 2015
Abstract: CDKN2A is a tumor suppressor gene critical in the cell cycle regulation. Little is known regarding the role of CDKN2A methylation in the pathogenesis of Burkitt lymphoma (BL). CDKN2A methylation was investigated using pyrosequencing in 51 tumor samples. p16(INK4a) mRNA and protein levels were measured using real-time PCR and immunohistochemistry, respectively. CDKN2A methylation was detectable in 72% cases. Nuclear expression of p16(INK4a) was not detected in 41% cases. There was an association between methylation and absence of CDKN2A mRNA (P=0.003). In conclusion, CDKN2A methylation occurs at a high frequency suggesting a role in BL pathogenesis and potential therapeutic implications.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Subjects :: Adolescent
Burkitt Lymphoma genetics
Cell Line, Tumor
Child, Preschool
Cyclin-Dependent Kinase Inhibitor p16 genetics
DNA, Neoplasm genetics
Female
Humans
Male
RNA, Messenger genetics
RNA, Neoplasm genetics
Burkitt Lymphoma metabolism
Cyclin-Dependent Kinase Inhibitor p16 biosynthesis
DNA Methylation
DNA, Neoplasm metabolism
Gene Expression Regulation, Neoplastic
RNA, Messenger biosynthesis
RNA, Neoplasm biosynthesis

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