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Seasonal Variation of Alkaloid Contents and Anti-Inflammatory Activity of Rhizoma coptidis Based on Fingerprints Combined with Chemometrics Methods.
- Source :
-
Journal of chromatographic science [J Chromatogr Sci] 2015 Aug; Vol. 53 (7), pp. 1131-9. Date of Electronic Publication: 2014 Dec 23. - Publication Year :
- 2015
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Abstract
- The fingerprints of Rhizoma coptidis (Huanglian) collected in different growing periods were established by high-performance liquid chromatography (HPLC), and the seasonal variations of six alkaloids, namely berberine, palmatine, jateorrhizine, coptisine, epiberberine and columbamine were assessed. In order to highlight the differences of their HPLC fingerprints, three chemometrics methods including Spearman's rank correlation, hierarchical clustering analysis (HCA) and principal component analysis were applied. The anti-inflammatory activity of R. coptidis collected in different developing stages was measured by NO inhibition assay. The relationship between HPLC fingerprints and anti-inflammatory activity named spectrum-effect relationship was investigated by partial least squares regression. The anti-inflammatory activity was positively correlated with the alkaloid contents. Berberine, palmatine, coptisine, jateorrhizine and epiberberine were the main anti-inflammatory components on the basis of the quantitative determination of the six alkaloids and spectrum-effect investigation. The collecting time of R. coptidis impacted the plant's alkaloid contents and its anti-inflammatory activity.<br /> (© The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Alkaloids pharmacology
Animals
Anti-Inflammatory Agents pharmacology
Cell Line
Cluster Analysis
Mice
Nitric Oxide antagonists & inhibitors
Principal Component Analysis
Seasons
Alkaloids analysis
Anti-Inflammatory Agents analysis
Chromatography, High Pressure Liquid methods
Coptis chemistry
Drugs, Chinese Herbal chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1945-239X
- Volume :
- 53
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of chromatographic science
- Publication Type :
- Academic Journal
- Accession number :
- 25540292
- Full Text :
- https://doi.org/10.1093/chromsci/bmu175