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Nodal signaling from the visceral endoderm is required to maintain Nodal gene expression in the epiblast and drive DVE/AVE migration.
- Source :
-
Developmental biology [Dev Biol] 2015 Apr 01; Vol. 400 (1), pp. 1-9. Date of Electronic Publication: 2014 Dec 20. - Publication Year :
- 2015
-
Abstract
- In the early mouse embryo, a specialized population of extraembryonic visceral endoderm (VE) cells called the distal VE (DVE) arises at the tip of the egg cylinder stage embryo and then asymmetrically migrates to the prospective anterior, recruiting additional distal cells. Upon migration these cells, called the anterior VE (AVE), establish the anterior posterior (AP) axis by restricting gastrulation-inducing signals to the opposite pole. The Nodal-signaling pathway has been shown to have a critical role in the generation and migration of the DVE/AVE. The Nodal gene is expressed in both the VE and in the pluripotent epiblast, which gives rise to the germ layers. Previous findings have provided conflicting evidence as to the relative importance of Nodal signaling from the epiblast vs. VE for AP patterning. Here we show that conditional mutagenesis of the Nodal gene specifically within the VE leads to reduced Nodal expression levels in the epiblast and incomplete or failed DVE/AVE migration. These results support a required role for VE Nodal to maintain normal levels of expression in the epiblast, and suggest signaling from both VE and epiblast is important for DVE/AVE migration.<br /> (Published by Elsevier Inc.)
- Subjects :
- Animals
Endoderm cytology
Galactosides
Genes, Reporter genetics
In Situ Hybridization
Indoles
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Mutagenesis
Nodal Protein genetics
Body Patterning physiology
Cell Movement physiology
Endoderm physiology
Gene Expression Regulation, Developmental physiology
Germ Layers metabolism
Nodal Protein metabolism
Signal Transduction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-564X
- Volume :
- 400
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 25536399
- Full Text :
- https://doi.org/10.1016/j.ydbio.2014.12.016