Back to Search
Start Over
LPS- and LTA-induced expression of IL-6 and TNF-α in neonatal and adult blood: role of MAPKs and NF-κB.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2014; Vol. 2014, pp. 283126. Date of Electronic Publication: 2014 Oct 30. - Publication Year :
- 2014
-
Abstract
- As nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) seem to be critical mediators in the inflammatory response, we studied the effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) on (a) the activation of NF-κB and MAPKs and (b) the expression of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) with or without the specific inhibitors of these intracellular signal transduction pathways in neonatal cord and adult blood. TNF-α and IL-6 concentrations showed a sharp increase in the supernatants of cord and adult whole blood after stimulation. TNF-α concentrations were significantly higher, whereas IL-6 concentrations were tendentially lower in adult blood after stimulation. Stimulation with LPS or LTA resulted in a significantly decreased activation of p38 MAPK in neonatal compared with adult blood. Although LTA failed to induce additional ERK1/2 phosphorylation, LPS stimulation mediated the moderately increased levels of activated ERK1/2 in neonatal monocytes. The addition of the p38 MAPK inhibitor SB202190 significantly decreased IL-6 and TNF-α production upon LPS or LTA stimulation. Furthermore, the inhibition of ERK1/2 was able to reduce LPS-stimulated TNF-α production in neonatal blood. We conclude that p38 MAPK as well as ERK1/2 phosphorylation is crucially involved in LPS activation and could explain the differences in early cytokine response between neonatal and adult blood.
- Subjects :
- Adult
Female
Fetal Blood metabolism
Flow Cytometry
Humans
Imidazoles chemistry
Infant, Newborn
JNK Mitogen-Activated Protein Kinases metabolism
MAP Kinase Signaling System
Monocytes cytology
Phosphorylation
Pregnancy
Pyridines chemistry
Signal Transduction
p38 Mitogen-Activated Protein Kinases metabolism
Gene Expression Regulation
Interleukin-6 blood
Lipopolysaccharides chemistry
NF-kappa B blood
Teichoic Acids chemistry
Tumor Necrosis Factor-alpha blood
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2014
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 25530682
- Full Text :
- https://doi.org/10.1155/2014/283126