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Myeloperoxidase oxidized LDL interferes with endothelial cell motility through miR-22 and heme oxygenase 1 induction: possible involvement in reendothelialization of vascular injuries.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2014; Vol. 2014, pp. 134635. Date of Electronic Publication: 2014 Nov 02. - Publication Year :
- 2014
-
Abstract
- Cardiovascular disease linked to atherosclerosis is the leading cause of death worldwide. Atherosclerosis is mainly linked to dysfunction in vascular endothelial cells and subendothelial accumulation of oxidized forms of LDL. In the present study, we investigated the role of myeloperoxidase oxidized LDL (Mox-LDL) in endothelial cell dysfunction. We studied the effect of proinflammatory Mox-LDL treatment on endothelial cell motility, a parameter essential for normal vascular processes such as angiogenesis and blood vessel repair. This is particularly important in the context of an atheroma plaque, where vascular wall integrity is affected and interference with its repair could contribute to progression of the disease. We investigated in vitro the effect of Mox-LDL on endothelial cells angiogenic properties and we also studied the signalling pathways that could be affected by analysing Mox-LDL effect on the expression of angiogenesis-related genes. We report that Mox-LDL inhibits endothelial cell motility and tubulogenesis through an increase in miR-22 and heme oxygenase 1 expression. Our in vitro data indicate that Mox-LDL interferes with parameters associated with angiogenesis. They suggest that high LDL levels in patients would impair their endothelial cell capacity to cope with a damaged endothelium contributing negatively to the progression of the atheroma plaque.
- Subjects :
- Animals
CHO Cells
Cell Movement
Cricetinae
Cricetulus
Disease Progression
Human Umbilical Vein Endothelial Cells cytology
Humans
Neovascularization, Pathologic
Plaque, Atherosclerotic metabolism
Signal Transduction
Vascular System Injuries metabolism
Wound Healing
Endothelial Cells cytology
Endothelium, Vascular metabolism
Heme Oxygenase-1 metabolism
Lipoproteins, LDL metabolism
MicroRNAs metabolism
Peroxidase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2014
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 25530680
- Full Text :
- https://doi.org/10.1155/2014/134635