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Serotonin receptor expression is dynamic in the liver during the transition period in Holstein dairy cows.
- Source :
-
Domestic animal endocrinology [Domest Anim Endocrinol] 2015 Apr; Vol. 51, pp. 65-73. Date of Electronic Publication: 2014 Nov 26. - Publication Year :
- 2015
-
Abstract
- Nonneuronal serotonin (5-HT) participates in glucose metabolism, but little is known regarding the actions of 5-HT in the liver during the transition period in dairy cattle. Here, we explore circulating patterns of 5-HT and characterize the hepatic 5-HT receptor and glucose transporter profiles around calving in multiparous Holstein dairy cows (n = 6, average lactation = 4 ± 1.9). Concentrations of serum 5-HT decreased on day -3 compared with -5 and -7 precalving (167.7 ± 80 vs 1511.1 ± 602 ng/mL). 5-HT nadir was on day -1 precalving and remained low postcalving (481.4 ± 49 ng/mL). Plasma glucose concentrations decreased precalving (P = 0.008) and were positively correlated with 5-HT during the precalving period (r = 0.55, P = 0.043). On day 1, postcalving hepatic messenger RNA expression of 5-HT1D, 2B, 3C, 6, and 7 receptors were decreased compared with day -7 (P < 0.048). The 5-HT3A and 5-HT3B decreased on day 7. The 5-HT2A increased on days 1 and 7 compared with -7 (P < 0.05). The 5-HT1F and 5-HT1A receptors were increased 2.5- and 3.8-fold on day 7, respectively, compared with days -7 and 1 (P < 0.046). The 5-HT5A was not detected, and 5-HT4 was detected on days -7 and 1 only. Expression of Glut-2,-5 and SGLT1 were decreased on days 1 and 7 compared with -7 (P < 0.05), whereas Glut-1 was increased on day 7 compared with -7 (P < 0.05). These results indicate that 5-HT could be important for liver glucose homeostasis possibly through receptor mediated signaling at specific times. Additional research is needed to further explore the functional role of these receptors in the liver during the transition from pregnancy to lactation.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Blood Glucose analysis
Dairying
Female
Glucose Transport Proteins, Facilitative genetics
Liver chemistry
Pregnancy
RNA, Messenger analysis
Serotonin analysis
Serotonin blood
Time Factors
Cattle physiology
Gene Expression
Lactation physiology
Liver metabolism
Parturition physiology
Receptors, Serotonin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0054
- Volume :
- 51
- Database :
- MEDLINE
- Journal :
- Domestic animal endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 25528206
- Full Text :
- https://doi.org/10.1016/j.domaniend.2014.11.005