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[Design of cell line stable expressing proteasomal subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag based on HEK293 cells].

Authors :
Kulichkova VA
Zaĭkova IuIa
Ermolaeva IuB
Vinogradova EV
Huang L
Tomilin AN
Barlev NA
Tsimokha AS
Source :
Tsitologiia [Tsitologiia] 2014; Vol. 56 (3), pp. 218-24.
Publication Year :
2014

Abstract

A stable cell line based on HEK293 cells that expresses proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag has been selected. This chimera was shown to be incorporated completely into proteolitic active proteasomes. The created cell line can be used for further fluorescent studies of proteasomes localization in the cell.

Details

Language :
Russian
ISSN :
0041-3771
Volume :
56
Issue :
3
Database :
MEDLINE
Journal :
Tsitologiia
Publication Type :
Academic Journal
Accession number :
25509418