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[Design of cell line stable expressing proteasomal subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag based on HEK293 cells].
- Source :
-
Tsitologiia [Tsitologiia] 2014; Vol. 56 (3), pp. 218-24. - Publication Year :
- 2014
-
Abstract
- A stable cell line based on HEK293 cells that expresses proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag has been selected. This chimera was shown to be incorporated completely into proteolitic active proteasomes. The created cell line can be used for further fluorescent studies of proteasomes localization in the cell.
- Subjects :
- Cell Engineering
Gene Expression
Green Fluorescent Proteins genetics
HEK293 Cells
Humans
Microscopy, Fluorescence
Proteasome Endopeptidase Complex genetics
Proteolysis
Recombinant Fusion Proteins genetics
Staining and Labeling
Trans-Activators genetics
Transfection
Founder Effect
Green Fluorescent Proteins metabolism
Plasmids chemistry
Proteasome Endopeptidase Complex metabolism
Recombinant Fusion Proteins metabolism
Trans-Activators metabolism
Subjects
Details
- Language :
- Russian
- ISSN :
- 0041-3771
- Volume :
- 56
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Tsitologiia
- Publication Type :
- Academic Journal
- Accession number :
- 25509418