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Development of a new DNA vaccine based on mycobacterial ESAT-6 antigen delivered by recombinant invasive Lactococcus lactis FnBPA+.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2015 Feb; Vol. 99 (4), pp. 1817-26. Date of Electronic Publication: 2014 Dec 14. - Publication Year :
- 2015
-
Abstract
- The use of the food-grade bacterium Lactococcus lactis as a vehicle for the oral delivery of DNA vaccine plasmids constitutes a promising strategy for vaccination. The delivery of DNA plasmids into eukaryotic cells is of critical importance for subsequent DNA expression and effectiveness of the vaccine. In this context, the use of the recombinant invasive L. lactis FnBPA+ (fibronectin-binding protein A) strain for the oral delivery of the eukaryotic expression vector vaccination using lactic acid bacteria (pValac), coding for the 6-kDa early secreted antigenic target (ESAT-6) gene of Mycobacterium tuberculosis, could represent a new DNA vaccine strategy against tuberculosis. To this end, the ESAT-6 sequence was cloned into the pValac vector; the L. lactis fibronectin-binding protein A (FnBPA)+ (pValac:ESAT-6) strain was obtained, and its immunological profile was checked in BALB/c mice. This strain was able to significantly increase interferon gamma (IFN-γ) production in spleen cells, showing a systemic T helper 1 (Th1) cell response. The mice also showed a significant increase in specific secretory immunoglobulin A (sIgA) production in colon tissue and fecal extracts. Thus, this is the first time that L. lactis has been used to deliver a plasmid DNA harboring a gene that encodes an antigen against tuberculosis through mucous membranes.
- Subjects :
- Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
Cloning, Molecular
Colon immunology
Feces chemistry
Immunoglobulin A, Secretory analysis
Interferon-gamma metabolism
Leukocytes, Mononuclear immunology
Mice, Inbred BALB C
Mycobacterium tuberculosis genetics
Plasmids
Spleen immunology
Tuberculosis Vaccines administration & dosage
Tuberculosis Vaccines genetics
Tuberculosis Vaccines isolation & purification
Vaccines, DNA genetics
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Vaccines, Synthetic isolation & purification
Antigens, Bacterial immunology
Bacterial Proteins immunology
Drug Carriers
Lactococcus lactis genetics
Mycobacterium tuberculosis immunology
Tuberculosis Vaccines immunology
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 99
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 25503506
- Full Text :
- https://doi.org/10.1007/s00253-014-6285-3