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The Pseudomonas syringae type III effector HopF2 suppresses Arabidopsis stomatal immunity.
- Source :
-
PloS one [PLoS One] 2014 Dec 11; Vol. 9 (12), pp. e114921. Date of Electronic Publication: 2014 Dec 11 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases required for regulating stomatal aperture, suggesting HopF2 may manipulate stomatal immunity. Here we report HopF2 can inhibit stomatal immunity independent of its ADP-ribosyltransferase activity. Transgenic expression of HopF2 in Arabidopsis inhibits stomatal closing in response to P. syringae and increases the virulence of surface inoculated P. syringae. Further, transgenic expression of HopF2 inhibits flg22 induced reactive oxygen species production. Intriguingly, ADP-ribosyltransferase activity is dispensable for inhibiting stomatal immunity and flg22 induced reactive oxygen species. Together, this implies HopF2 may be a bifunctional T3SE with ADP-ribosyltransferase activity required for inhibiting apoplastic immunity and an independent function required to inhibit stomatal immunity.
- Subjects :
- ADP Ribose Transferases metabolism
Arabidopsis immunology
Host-Pathogen Interactions immunology
Plant Stomata genetics
Plants, Genetically Modified
Proteomics
Pseudomonas syringae metabolism
Pseudomonas syringae pathogenicity
Type III Secretion Systems genetics
Type III Secretion Systems metabolism
ADP Ribose Transferases genetics
Arabidopsis genetics
Bacterial Proteins genetics
Host-Pathogen Interactions genetics
Plant Immunity genetics
Plant Stomata immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25503437
- Full Text :
- https://doi.org/10.1371/journal.pone.0114921