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Arteries are formed by vein-derived endothelial tip cells.
- Source :
-
Nature communications [Nat Commun] 2014 Dec 15; Vol. 5, pp. 5758. Date of Electronic Publication: 2014 Dec 15. - Publication Year :
- 2014
-
Abstract
- Tissue vascularization entails the formation of a blood vessel plexus, which remodels into arteries and veins. Here we show, by using time-lapse imaging of zebrafish fin regeneration and genetic lineage tracing of endothelial cells in the mouse retina, that vein-derived endothelial tip cells contribute to emerging arteries. Our movies uncover that arterial-fated tip cells change migration direction and migrate backwards within the expanding vascular plexus. This behaviour critically depends on chemokine receptor cxcr4a function. We show that the relevant Cxcr4a ligand Cxcl12a selectively accumulates in newly forming bone tissue even when ubiquitously overexpressed, pointing towards a tissue-intrinsic mode of chemokine gradient formation. Furthermore, we find that cxcr4a mutant cells can contribute to developing arteries when in association with wild-type cells, suggesting collective migration of endothelial cells. Together, our findings reveal specific cell migratory behaviours in the developing blood vessel plexus and uncover a conserved mode of artery formation.
- Subjects :
- Animal Fins blood supply
Animal Fins cytology
Animal Fins growth & development
Animal Fins metabolism
Animals
Animals, Genetically Modified
Arteries cytology
Arteries metabolism
Cell Lineage genetics
Cell Movement
Chemokine CXCL12 genetics
Chemokine CXCL12 metabolism
Endothelial Cells cytology
Endothelium, Vascular cytology
Endothelium, Vascular growth & development
Gene Expression Regulation, Developmental
Mice
Receptors, CXCR4 genetics
Retina cytology
Retina growth & development
Retina metabolism
Signal Transduction
Time-Lapse Imaging
Veins cytology
Veins metabolism
Video Recording
Zebrafish genetics
Zebrafish metabolism
Zebrafish Proteins genetics
Arteries growth & development
Endothelial Cells metabolism
Endothelium, Vascular metabolism
Neovascularization, Physiologic
Receptors, CXCR4 metabolism
Veins growth & development
Zebrafish Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 25502622
- Full Text :
- https://doi.org/10.1038/ncomms6758