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Reduced Ki67 Staining in the Postmortem State Calls Into Question Past Conclusions About the Lack of Turnover of Adult Human β-Cells.
- Source :
-
Diabetes [Diabetes] 2015 May; Vol. 64 (5), pp. 1698-702. Date of Electronic Publication: 2014 Dec 08. - Publication Year :
- 2015
-
Abstract
- Some report that adult human β-cells do not replicate, but we postulate this assumption is erroneous due a postmortem decline in replication markers such as Ki67. Our earlier report showed that Ki67-marked β-cells were rarely found in human cadaveric pancreases but were in the range of 0.2-0.5% in human islets transplanted into mice. This study subjected 4-week-old mice to autopsy conditions that typically occur with humans. Mice were killed, left at room temperature for 3 h, and then placed at 4°C for 3, 9, or 21 h. There was a rapid marked fall in Ki67 staining of β-cells compared with those fixed immediately. Values at death were 6.9 ± 0.9% (n = 6) after a 24-h fast, 4.1 ± 0.9% (n = 6) at 3 h room temperature, 2.7 ± 0.7% (n = 5) at 6 h, 1.6 ± 0.6% (n = 5) at 12 h, and 2.9 ± 0.8% (n = 5) at 24 h. Similar postmortem conditions in newborn pigs resulted in very similar declines in Ki67 staining of their β-cells. These data support the hypothesis that conclusions on the lack of replication of adult human β-cells are incorrect and suggest that adult human β-cells replicate at a low but quantitatively meaningful rate.<br /> (© 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.)
- Subjects :
- Animals
Animals, Newborn
Biomarkers
Gene Expression Regulation physiology
Humans
Ischemia
Mice
Postmortem Changes
Swine
Temperature
Insulin-Secreting Cells cytology
Insulin-Secreting Cells physiology
Islets of Langerhans Transplantation physiology
Ki-67 Antigen chemistry
Ki-67 Antigen metabolism
Staining and Labeling
Subjects
Details
- Language :
- English
- ISSN :
- 1939-327X
- Volume :
- 64
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Diabetes
- Publication Type :
- Academic Journal
- Accession number :
- 25488899
- Full Text :
- https://doi.org/10.2337/db14-1675