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Semi-automated selection of cryo-EM particles in RELION-1.3.
- Source :
-
Journal of structural biology [J Struct Biol] 2015 Feb; Vol. 189 (2), pp. 114-22. Date of Electronic Publication: 2014 Dec 06. - Publication Year :
- 2015
-
Abstract
- The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.<br /> (Copyright © 2014 The Author. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1095-8657
- Volume :
- 189
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of structural biology
- Publication Type :
- Academic Journal
- Accession number :
- 25486611
- Full Text :
- https://doi.org/10.1016/j.jsb.2014.11.010