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Click N-benzyl iminodiacetic acid: novel silica-based tridentate zwitterionic stationary phase for hydrophilic interaction liquid chromatography.
- Source :
-
Talanta [Talanta] 2015 Jan; Vol. 132, pp. 137-45. Date of Electronic Publication: 2014 Sep 06. - Publication Year :
- 2015
-
Abstract
- Iminodiacetic acid (IDA) is dicarboxylic acid amine, which may produce stronger interaction with polar or charged compounds than bidentate α,β-amino acid. In this article, a novel type of tridentate zwitterionic HILIC stationary phase was prepared by covalently bonding N-benzyl IDA on silica gel via copper(I) catalyzed Huisgen azide-alkyne 1,3-dipolar cycloaddition (CuAAC). The structure of this stationary phase and all related intermediates was confirmed by NMR, FT-IR, MS spectrum and elemental analysis. The new stationary phase showed good HILIC characteristics and high column efficiency (the theoretical plate number is up to 44000 plates m(-1) in the case of guanosine) in the application of separation of polar compounds, including organic acids, organic bases, as well as highly polar and hydrophilic compounds, such as cephalosporins and carbapenems. Most of them displayed good peak shape and selectivity.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Alkynes chemistry
Azides chemistry
Benzoates isolation & purification
Click Chemistry
Cycloaddition Reaction
Hydrophobic and Hydrophilic Interactions
Purines isolation & purification
Pyrimidines isolation & purification
Solutions
Sulfadiazine isolation & purification
Carbapenems isolation & purification
Cephalosporins isolation & purification
Chromatography, Liquid methods
Imino Acids chemistry
Silica Gel chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 132
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 25476290
- Full Text :
- https://doi.org/10.1016/j.talanta.2014.08.077