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Coordinated V gamma and V delta gene segment rearrangements in human T cell receptor gamma/delta+ lymphocytes.
- Source :
-
European journal of immunology [Eur J Immunol] 1989 Jul; Vol. 19 (7), pp. 1261-5. - Publication Year :
- 1989
-
Abstract
- Monoclonal antibodies (mAb) were used to characterize a panel (n = 46) of T cell receptor (TcR) gamma/delta+ T cell clones. Three of these antibodies have been described to react with specific variable region-encoded protein products and can therefore be used to detect functional gene rearrangements. The majority of peripheral blood-derived clones (43 out of 45) expressed the epitopes recognized by mAb BB3, encoded by the V delta 2 gene segment and mAb Ti gamma A, encoded by the V gamma 9 gene segment. These clones lacked the antigenic determinant recognized by mAb delta-TCS-1, encoded by the V delta 1 gene segment. The other two peripheral blood-derived clones and an ascites-derived clone were Ti gamma A-, BB3- and delta-TCS-1+. Biochemical analysis revealed that all Ti gamma A+, BB3+ T cell clones expressed the disulfide-linked form of the receptor. The two peripheral blood-derived delta-TCS-1+ T cell clones expressed the nondisulfide-linked form whereas the ascites-derived delta-TCS-1+ clone, AK119 expressed the disulfide-linked form of the TcR gamma/delta heterodimer. This indicates that V delta 1-encoded delta chains can be associated either with a C gamma 1- or a C gamma 2-encoded gamma chain. The preferential use of certain V gamma and V delta gene segments suggests the existence of a limited combinatorial diversity in TcR gamma/delta heterodimers, i.e. Ti gamma A+ (V gamma 9), BB3+ (V delta 2) and delta-TCS-1- disulfide-linked heterodimers and Ti gamma A-, BB3- and delta-TCS-1+ (V delta 1) disulfide- or non disulfide-linked forms.
- Subjects :
- Antibodies, Monoclonal immunology
Blotting, Southern
Disulfides
Humans
Precipitin Tests
Receptors, Antigen, T-Cell immunology
Receptors, Antigen, T-Cell, gamma-delta
Gene Rearrangement, T-Lymphocyte
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
Receptors, Antigen, T-Cell genetics
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2547624
- Full Text :
- https://doi.org/10.1002/eji.1830190717