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Enrichment of CD56(dim)KIR + CD57 + highly cytotoxic NK cells in tumour-infiltrated lymph nodes of melanoma patients.
- Source :
-
Nature communications [Nat Commun] 2014 Dec 04; Vol. 5, pp. 5639. Date of Electronic Publication: 2014 Dec 04. - Publication Year :
- 2014
-
Abstract
- An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumour-infiltrated (TILN) and tumour-free ipsilateral lymph nodes (TFLN) of the same patients. We show an expansion of CD56(dim)CD57(dim)CD69 + CCR7 +KIR+ NK cells in TILN. TILN NK cells display robust cytotoxic activity against autologous melanoma cells. In the blood of metastatic melanoma patients, the frequency of NK cells expressing the receptors for CXCL8 receptor is increased compared with healthy subjects, and blood NK cells also express the receptors for CCL2 and IL-6. These factors are produced in high amount in TILN and in vitro switch the phenotype of blood NK cells from healthy donors to the phenotype associated with TILN. Our data suggest that the microenvironment of TILN generates and/or recruits a particularly effective NK cell subset.
- Subjects :
- Adult
Aged
Antigens, CD metabolism
Antigens, Differentiation, T-Lymphocyte metabolism
CD56 Antigen metabolism
CD57 Antigens metabolism
Female
Humans
Killer Cells, Natural metabolism
Lectins, C-Type metabolism
Lymph Nodes pathology
Lymphatic Metastasis
Male
Melanoma pathology
Middle Aged
Receptors, CCR7 metabolism
Receptors, Interleukin-6 metabolism
Receptors, KIR2DL2 metabolism
Receptors, KIR2DL3 metabolism
Skin Neoplasms pathology
Tumor Microenvironment immunology
Killer Cells, Natural immunology
Lymph Nodes immunology
Melanoma immunology
Skin Neoplasms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 25472612
- Full Text :
- https://doi.org/10.1038/ncomms6639