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[Molecular identification of 15 species of Ilex genus based on ITS sequence analysis ].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2014 Jun; Vol. 37 (6), pp. 974-6. - Publication Year :
- 2014
-
Abstract
- Objective: To establish molecular method for identification of Ilex plants using the internal transcribed spacer(ITS) bar- code.<br />Methods: DNA was extracted using centrifugal columnar kits. The ITS sequences of all experimental samples were amplified and sequenced using a pair of universal primers. The alignment and the phylogenetic tree were performed in MEGA5. 0.<br />Results: The length of ITS sequence in each species was 759 bp,in which there were 216 sites discrepancy.<br />Conclusion: DNA barcoding technology based on ITS sequence can be used to identify plants of Ilex genus.
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 37
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 25470961