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A fast and simple solid phase microextraction coupled with gas chromatography-triple quadrupole mass spectrometry method for the assay of urinary markers of glutaric acidemias.

Authors :
Naccarato A
Gionfriddo E
Elliani R
Sindona G
Tagarelli A
Source :
Journal of chromatography. A [J Chromatogr A] 2014 Dec 12; Vol. 1372C, pp. 253-259. Date of Electronic Publication: 2014 Oct 30.
Publication Year :
2014

Abstract

The analysis of characteristic urinary acidic markers such as glutaric, 3-hydroxyglutaric, 2-hydroxyglutaric, adipic, suberic, sebacic, ethylmalonic, 3-hydroxyisovaleric and isobutyric acid constitutes the recommended follow-up testing procedure for glutaric acidemia type 1 (GA-1) and type 2 (GA-2). The goal of the work herein presented is the development of a fast and simple method for the quantification of these biomarkers in human urine. The proposed analytical approach is based on the use of solid phase microextraction (SPME) combined with gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS) afterward a rapid derivatization of acidic moieties by propyl chloroformate, propanol and pyridine. Trueness and precision of the proposed protocol, tested at 5, 30 and 80mgl <superscript>-1</superscript> , provided satisfactory values: recoveries were in the range between 72% and 116% and the relative standard deviations (RSD%) were between 0.9% and 18% (except for isobutyric acid at 5mgl <superscript>-1</superscript> ). The LOD values achieved by the proposed method ranged between 1.0 and 473μgl <superscript>-1</superscript> .<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3778
Volume :
1372C
Database :
MEDLINE
Journal :
Journal of chromatography. A
Publication Type :
Academic Journal
Accession number :
25468502
Full Text :
https://doi.org/10.1016/j.chroma.2014.10.069