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Immobilizing PEO-PPO-PEO triblock copolymers on hydrophobic surfaces and its effect on protein and platelet: a combined study using QCM-D and DPI.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2014 Nov 01; Vol. 123, pp. 892-9. Date of Electronic Publication: 2014 Oct 28. - Publication Year :
- 2014
-
Abstract
- Dual polarization interferometry was used to monitor the immobilization dynamics of four Pluronics on hydrophobic surfaces and to elucidate the effect of Pluronic conformation on protein adsorption. The proportion of hydrophobic chain segments and not the length of the hydrophobic chain can influence the chain densities of the Pluronics. The immobilized densities of the Pluronics resulted from competition between the hydration of polyethylene oxide (PEO) in the aqueous solution and the hydrophobic interaction of polypropylene oxide on the substrate. P-123 obtained the largest graft mass (2.89±0.25 ng/mm2) because of the dominant effect of hydrophobic interactions. Hydrophobic segments of P-123 were anchored slowly and step-wise on the C18 substrate. P-123 exhibited the largest hydrophobic chain segment proportion (propylene oxide/ethylene oxide=3.63) and formed a brush chain conformation, indicating excellent protein and platelet resistance. The result of quartz crystal microbalance with dissipation further confirmed that the PEO conformation in P-123 on the substrate exhibited a relatively extended brush chain, and that L-35 showed relatively loose and pancake-like structures. The PEO in P-123 regulated the conformation to maintain the native conformation and resist the adsorption of bovine serum albumin (BSA). Thus, the hemocompatibilities of the immobilized Pluronics were influenced by the proportion of hydrophobic chain segments and their PEO conformations.<br /> (Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Blood Platelets drug effects
Cattle
Hydrophobic and Hydrophilic Interactions
Platelet Adhesiveness drug effects
Poloxamer
Polyethylene Glycols adverse effects
Polymers adverse effects
Propylene Glycols adverse effects
Serum Albumin, Bovine drug effects
Polyethylene Glycols chemistry
Polymers chemistry
Propylene Glycols chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 123
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 25466461
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2014.10.038