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Characterization of sakA gene from pathogenic dimorphic fungus Penicillium marneffei.
- Source :
-
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2015 Jan; Vol. 305 (1), pp. 65-74. Date of Electronic Publication: 2014 Nov 11. - Publication Year :
- 2015
-
Abstract
- Eukaryotes utilize stress activated protein kinase (SAPK) pathways to adapt to environmental stress, including heat, osmotic, oxidative or nutrient stresses. Penicillium marneffei (Talaromyces marneffei), the dimorphic pathogenic fungus that can cause disseminated mycosis in HIV-infected patients, has to encounter various types of stresses both outside and inside host cells. However, the strategies used by this fungus in response to these stresses are still unclear. In this report, the stress-activated kinase (sakA) gene of P. marneffei was characterized and the roles of this gene on various stress conditions were studied. The sakA gene deletion mutant was constructed using the split marker method. The phenotypes and sensitivities to varieties of stresses, including osmotic, oxidative, heat and cell wall stresses of the deletion mutant were compared with the wild type and the sakA complemented strains. Results demonstrated that the P. marneffei sakA gene encoded a putative protein containing TXY phosphorylation lip found in the stress high osmolarity glycerol 1 (Hog1)/Spc1/p38 MAPK family, and that this gene was involved not only in tolerance against oxidative and heat stresses, but also played a role in asexual development, chitin deposition, yeast cell generation in vitro and survival inside mouse and human macrophages.<br /> (Copyright © 2014 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Cell Line
Chitin metabolism
DNA, Fungal chemistry
DNA, Fungal genetics
Gene Deletion
Genetic Complementation Test
Hot Temperature
Humans
Macrophages microbiology
Mice
Microbial Viability drug effects
Microbial Viability radiation effects
Molecular Sequence Data
Osmotic Pressure
Oxidative Stress
Penicillium drug effects
Penicillium genetics
Penicillium radiation effects
Protein Kinases genetics
Sequence Analysis, DNA
Penicillium physiology
Protein Kinases metabolism
Stress, Physiological
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0607
- Volume :
- 305
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of medical microbiology : IJMM
- Publication Type :
- Academic Journal
- Accession number :
- 25466206
- Full Text :
- https://doi.org/10.1016/j.ijmm.2014.11.003