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Nuclear export of cutaneous HPV8 E7 oncoprotein is mediated by a leucine-rich nuclear export signal via a CRM1 pathway.
Nuclear export of cutaneous HPV8 E7 oncoprotein is mediated by a leucine-rich nuclear export signal via a CRM1 pathway.
- Source :
-
Virology [Virology] 2015 Jan 01; Vol. 474, pp. 28-33. Date of Electronic Publication: 2014 Nov 09. - Publication Year :
- 2015
-
Abstract
- We recently determined that the nuclear import of cutaneous beta genus HPV8 E7 oncoprotein it is mediated by its zinc-binding domain via direct hydrophobic interactions with the FG nucleoporins Nup62 and Nup153 (Onder and Moroianu, 2014). Here we investigated the nuclear export of HPV8 E7 oncoprotein using confocal microscopy after transfections of HeLa cells with EGFP-8cE7 and mutant plasmids and treatment with Ratjadone A nuclear export inhibitor. We determined that HPV8 E7 contains a leucine-rich nuclear export signal (NES), 76IRTFQELLF84, within its zinc-binding domain that mediates its nuclear export via a CRM1 pathway. We found that HPV8 E7 interacts with CRM1 and that the hydrophobic amino acid residues I76, F79 and L82 of the NES are essential for this interaction and for nuclear export of HPV8 E7 oncoprotein.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Active Transport, Cell Nucleus
Amino Acid Substitution
Betapapillomavirus genetics
Betapapillomavirus pathogenicity
HeLa Cells
Host-Pathogen Interactions genetics
Host-Pathogen Interactions physiology
Humans
Mutagenesis, Site-Directed
Nuclear Export Signals genetics
Nuclear Export Signals physiology
Papillomavirus E7 Proteins chemistry
Papillomavirus E7 Proteins genetics
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Skin Neoplasms etiology
Skin Neoplasms virology
Exportin 1 Protein
Betapapillomavirus metabolism
Karyopherins metabolism
Papillomavirus E7 Proteins metabolism
Receptors, Cytoplasmic and Nuclear metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 474
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 25463601
- Full Text :
- https://doi.org/10.1016/j.virol.2014.10.012