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A triticale water-deficit-inducible phytocystatin inhibits endogenous cysteine proteinases in vitro.
- Source :
-
Journal of plant physiology [J Plant Physiol] 2015 Feb 01; Vol. 174, pp. 161-5. Date of Electronic Publication: 2014 Oct 16. - Publication Year :
- 2015
-
Abstract
- Water-deficit is accompanied by an increase in proteolysis. Phytocystatins are plant inhibitors of cysteine proteinases that belong to the papain and legumain family. A cDNA encoding the protein inhibitor TrcC-8 was identified in the vegetative organs of triticale. In response to water-deficit, increases in the mRNA levels of TrcC-8 were observed in leaf and root tissues. Immunoblot analysis indicated that accumulation of the TrcC-8 protein occurred after 72h of water-deficit in the seedlings. Using recombinant protein, inhibitory activity of TrcC-8 against cysteine proteases from triticale and wheat tissues was analyzed. Under water-deficit conditions, there are increases in cysteine proteinase activities in both plant tissues. The cysteine proteinase activities were inhibited by addition of the recombinant TrcC-8 protein. These results suggest a potential role for the triticale phytocystatin in modulating cysteine proteinase activities during water-deficit conditions.<br /> (Copyright © 2014 Elsevier GmbH. All rights reserved.)
- Subjects :
- Droughts
Edible Grain genetics
Gene Expression Regulation, Plant
Immunoblotting
Plant Proteins genetics
Plant Proteins metabolism
Proteolysis
Stress, Physiological genetics
Triticum metabolism
Cystatins metabolism
Cysteine Proteases metabolism
Edible Grain enzymology
Edible Grain physiology
Water metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-1328
- Volume :
- 174
- Database :
- MEDLINE
- Journal :
- Journal of plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 25462979
- Full Text :
- https://doi.org/10.1016/j.jplph.2014.09.014