Prediction by 31P-NMR of the irreversibility of ischemic injury in rat skeletal muscle after ligation of the femoral artery.

Rat leg muscles, rendered ischaemic 1 hour previously by ligation of the femoral artery, were submitted to 20 minutes exercise by electrical stimulation of the sciatic nerve. 31P-NMR spectroscopy was used to monitor the changes in high-energy phosphate content of the muscles before, during, and after exercise. Fifteen of the 35 studied muscles evolved toward total necrosis, whereas the others showed signs of recovery over a 2-5-hour postexercise period. Those muscles which did not subsequently recover contained significantly more inorganic phosphate (Pi) at rest (before exercise) than those which recovered. It is suggested that under acute ischaemic conditions the Pi level at rest is correlated with the extent of blood flow restriction and can be used to predict the severity of the ischemia.