A new method for polyhydroxyalkanoate (PHA) accumulating bacteria selection under physical selective pressure.

Authors :: Chen Z
Guo Z
Wen Q
Huang L
Bakke R
Du M
Source :: International journal of biological macromolecules [Int J Biol Macromol] 2015 Jan; Vol. 72, pp. 1329-34. Date of Electronic Publication: 2014 Oct 22.
Publication Year :: 2015
Abstract: An aerobic dynamic discharge (ADD) process involving physical selective pressure to favor the accumulation of polyhydroxyalkanoates (PHAs) accumulating bacteria in microbial mixed cultures (MMCs) is proposed and evaluated in this study. Sequencing batch reactor PHA production in the ADD mode was compared to the conventional aerobic dynamic feeding (ADF) mode. Results showed that the cultures selected under the ADD mode showed a better PHA producing potential than ADF mode cultures in terms of both maximum PHA content and selection time, explained by altered physical selective pressure. The maximum PHA content of 74.16 (± 0.03)% and PHA yield of 0.72 (± 0.07) C mol PHA/C mol Ac were achieved after 30 days of the ADD operation, which were much higher than those obtained under the same organic loading rate in the ADF operation. The ADD mode is a promising method for quick selection of a strong-PHA accumulating culture.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Subjects :: Batch Cell Culture Techniques
Bioreactors microbiology
Kinetics
Time Factors
Bacteria isolation & purification
Biotechnology methods
Polyhydroxyalkanoates metabolism
Pressure

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