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Folding and stability of integral membrane proteins in amphipols.

Authors :
Kleinschmidt JH
Popot JL
Source :
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2014 Dec 15; Vol. 564, pp. 327-43. Date of Electronic Publication: 2014 Oct 30.
Publication Year :
2014

Abstract

Amphipols (APols) are a family of amphipathic polymers designed to keep transmembrane proteins (TMPs) soluble in aqueous solutions in the absence of detergent. APols have proven remarkably efficient at (i) stabilizing TMPs, as compared to detergent solutions, and (ii) folding them from a denatured state to a native, functional one. The underlying physical-chemical mechanisms are discussed.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-0384
Volume :
564
Database :
MEDLINE
Journal :
Archives of biochemistry and biophysics
Publication Type :
Academic Journal
Accession number :
25449655
Full Text :
https://doi.org/10.1016/j.abb.2014.10.013