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Pharmacological and signalling properties of a D2-like dopamine receptor (Dop3) in Tribolium castaneum.
- Source :
-
Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2015 Jan; Vol. 56, pp. 9-20. Date of Electronic Publication: 2014 Nov 15. - Publication Year :
- 2015
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Abstract
- Dopamine is an important neurotransmitter in the central nervous system of vertebrates and invertebrates. Despite their evolutionary distance, striking parallels exist between deuterostomian and protostomian dopaminergic systems. In both, signalling is achieved via a complement of functionally distinct dopamine receptors. In this study, we investigated the sequence, pharmacology and tissue distribution of a D2-like dopamine receptor from the red flour beetle Tribolium castaneum (TricaDop3) and compared it with related G protein-coupled receptors in other invertebrate species. The TricaDop3 receptor-encoding cDNA shows considerable sequence similarity with members of the Dop3 receptor class. Real time qRT-PCR showed high expression in both the central brain and the optic lobes, consistent with the role of dopamine as neurotransmitter. Activation of TricaDop3 expressed in mammalian cells increased intracellular Ca(2+) signalling and decreased NKH-477 (a forskolin analogue)-stimulated cyclic AMP levels in a dose-dependent manner. We studied the pharmacological profile of the TricaDop3 receptor and demonstrated that the synthetic vertebrate dopamine receptor agonists, 2 - amino- 6,7 - dihydroxy - 1,2,3,4 - tetrahydronaphthalene hydrobromide (6,7-ADTN) and bromocriptine acted as agonists. Methysergide was the most potent of the antagonists tested and showed competitive inhibition in the presence of dopamine. This study offers important information on the Dop3 receptor from Tribolium castaneum that will facilitate functional analyses of dopamine receptors in insects and other invertebrates.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
CHO Cells
Cricetulus
Cyclic AMP metabolism
Dopamine Agonists pharmacology
Dopamine Antagonists pharmacology
HEK293 Cells
Humans
Molecular Sequence Data
Real-Time Polymerase Chain Reaction
Receptors, Dopamine analysis
Receptors, G-Protein-Coupled metabolism
Sequence Analysis, Protein
Signal Transduction
Tribolium genetics
Receptors, Dopamine metabolism
Tribolium drug effects
Tribolium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0240
- Volume :
- 56
- Database :
- MEDLINE
- Journal :
- Insect biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 25449128
- Full Text :
- https://doi.org/10.1016/j.ibmb.2014.11.002