Synthesis and biological evaluation of nandrolone-bodipy conjugates.

Authors :: Jurášek M
Rimpelová S
Pavlíčková V
Ruml T
Lapčík O
Drašar PB
Source :: Steroids [Steroids] 2015 May; Vol. 97, pp. 62-6. Date of Electronic Publication: 2014 Nov 01.
Publication Year :: 2015
Abstract: Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)