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CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
- Source :
-
Stem cells (Dayton, Ohio) [Stem Cells] 2015 Mar; Vol. 33 (3), pp. 976-87. - Publication Year :
- 2015
-
Abstract
- Previous studies have predicted that reciprocal activation of GATA-1 and PU.1 regulates myelo-erythroid versus myelo-lymphoid lineage commitment in early hematopoiesis. Such PU.1-activating myelo-lymphoid progenitors exist within the lymphoid-primed multipotent progenitor (LMPP) population at the primitive Lineage(-) Sca-1(+) c-Kit(+) (LSK) stage. We here show that the counterpart of GATA-1-activating myelo-erythroid progenitor resides also at the LSK stage, expressing CD41 at a high level. Purified CD41(hi) LSK cells showed exceedingly strong and prolonged myelo-erythroid-restricted reconstitution, and primed myelo-erythroid gene expression with a more primitive molecular signature as compared to the original common myeloid progenitor (CMP). The CD41(hi) LSK cells more strongly contributed to emergent and malignant myelopoiesis than LMPPs, and produced the original CMP by downregulating Sca-1 and CD41, suggesting that they are the earliest CMPs. Thus, the hematopoietic developmental map should be revised by integrating the primary branchpoint comprised of the new, isolatable CD41(hi) CMP and the LMPP populations.<br /> (© 2014 AlphaMed Press.)
- Subjects :
- Animals
Cell Differentiation physiology
Cell Lineage
Cells, Cultured
Gene Expression Profiling
Hematopoietic Stem Cells metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myeloid Progenitor Cells metabolism
Hematopoiesis physiology
Hematopoietic Stem Cells cytology
Leukemia, Myeloid pathology
Myeloid Progenitor Cells cytology
Platelet Membrane Glycoprotein IIb metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1549-4918
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Stem cells (Dayton, Ohio)
- Publication Type :
- Academic Journal
- Accession number :
- 25446279
- Full Text :
- https://doi.org/10.1002/stem.1906