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Mode of action of Bacillus licheniformis pectin methylesterase on highly methylesterified and acetylated pectins.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2015 Jan 22; Vol. 115, pp. 540-50. Date of Electronic Publication: 2014 Sep 19. - Publication Year :
- 2015
-
Abstract
- A gene encoding a putative pectinesterase from Bacillus licheniformis DSM13 was cloned and expressed in Escherichia coli. The resulting recombinant enzyme (BliPME) was purified and characterized as a pectin methylesterase. The enzyme showed maximum activity at pH 8.0 and 50°C. BliPME is able to release up to 100% of the methylesters from lime pectin (DM 34-76→DM 0) and up to 73% of all methylesters from SBPs (DM 30-73→DM 14). BliPME efficiently de-methylesterifies lemon pectins and SBPs in a blockwise manner and is quite tolerant towards the acetyl groups present within the SBPs. Detailed analysis of the BliPME-modified pectins using HILIC-MSn and the classical calcium reactivity measurement showed that the enzyme generates pectins with low methylesterification (lime and SBP) and high acetyl content (SBP) while creating blocks of nonmethylesterified galacturonic acid residues. The high activity of BliPME towards highly methylesterified and acetylated pectins makes this novel esterase more efficient in removing methylesters from highly esterified beet pectin compared to other PMEs, e.g. Aspergillus niger PME.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Acetylation
Amino Acid Sequence
Bacterial Proteins genetics
Bacterial Proteins isolation & purification
Bacterial Proteins metabolism
Beta vulgaris
Carboxylic Ester Hydrolases genetics
Carboxylic Ester Hydrolases isolation & purification
Carboxylic Ester Hydrolases metabolism
Escherichia coli genetics
Esterification
Molecular Sequence Data
Sequence Alignment
Sequence Homology
Bacillus enzymology
Bacterial Proteins chemistry
Carboxylic Ester Hydrolases chemistry
Pectins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 115
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 25439930
- Full Text :
- https://doi.org/10.1016/j.carbpol.2014.09.016