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[Comparative evaluation of stx-PCR, Vero cell assay and Verotoxin enzyme imminoassay for detection of Shiga toxin-producing Escherichia coli].
- Source :
-
Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2014 Sep; Vol. 43 (5), pp. 797-804. - Publication Year :
- 2014
-
Abstract
- Objective: To evaluate the suitability of stx-PCR, Vero cell assay and commercial enzyme immunoassay for detection of Shiga toxin Escherichia coli and to compare sensitivity and specificity of three different methods for detection of Shiga toxin-producing Escherichia coli.<br />Methods: Using stx-PCR, Vero cell assay and commercial enzyme immunoassay to detect 35 Escherichia coli reference strains and 45 strains isolated from food.<br />Results: The three methods all had good specificity. 31 strains gave positive reaction in the Vero cell assay and in the stx-PCR. The consistency between the Vero cell assay and stx-PCR was 100%. Only 38 strains can be detected by commercial enzyme immunoassay.<br />Conclusion: stx-PCR method can serve as a routine rapid detection method in the laboratory. Vero cell assay is recommended to be the gold standard to determine whether the bacteria had the functionally active toxin. Commercial kit was suitable for preliminary rapid detection during clinical testing and outbreaks of food-borne disease.
- Subjects :
- Animals
Chlorocebus aethiops
Escherichia coli
Escherichia coli Infections diagnosis
Foodborne Diseases
Humans
Reproducibility of Results
Sensitivity and Specificity
Shiga Toxins analysis
Shiga Toxins genetics
Shiga-Toxigenic Escherichia coli genetics
Vero Cells
Enzyme-Linked Immunosorbent Assay methods
Escherichia coli Infections microbiology
Food Microbiology
Polymerase Chain Reaction methods
Shiga Toxins biosynthesis
Shiga-Toxigenic Escherichia coli isolation & purification
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-8020
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Wei sheng yan jiu = Journal of hygiene research
- Publication Type :
- Academic Journal
- Accession number :
- 25438538