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[Correlation between five RNA markers of rat's skin and PMI at different temperatures].
- Source :
-
Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2014 Aug; Vol. 30 (4), pp. 245-9. - Publication Year :
- 2014
-
Abstract
- Objective: To explore the correlation between postmortem interval (PMI) and five RNA markers of rat's skin--β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S ribosomal RNA(18S rRNA), 5S ribosomal RNA (5S rRNA), and microRNA-203 (miR-203), at different temperatures.<br />Methods: Eighteen SD rats were randomly divided into three environmental temperature groups: 4 °C, 15 °C and 35 °C, respectively. Skin samples were taken at 11 time points from 0 h to 120 h post-mortem. The total RNA was extracted from the skin samples and the five RNA levels were detected by real-time fluorescent quantitative PCR. Proper internal reference was selected by geNorm software. Regression analysis of the RNA markers was conducted by GraphPad software.<br />Results: 5S rRNA and miR-203 were most suitable internal references. A good linear relationship between PMI and RNA levels (β-actin and GAPDH) was observed in two groups (4 °C and 15 °C), whereas the S type curve relationship between the expression levels of the two markers (β-actin and GAPDH) and PMI was observed in the 35 °C group. The partial linear relationship between 18S rRNA and PMI was observed in the groups (15 °C and 35 °C).<br />Conclusion: Skin could be a suitable material for extracting RNA. The RNA expression levels of β-actin and GAPDH correlate well with PMI, and these RNA markers of skin tissue could be additional indice for the estimation of PMI.
Details
- Language :
- Chinese
- ISSN :
- 1004-5619
- Volume :
- 30
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Fa yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 25434083