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Characterization of a protein fraction containing cytochromes b and c1 from mitochondria of Leishmania tarentolae.
- Source :
-
Experimental parasitology [Exp Parasitol] 1989 May; Vol. 68 (4), pp. 443-9. - Publication Year :
- 1989
-
Abstract
- A soluble red band fraction was obtained from Leishmania tarentolae cells by sucrose gradient sedimentation of a Triton X-100 lysate. Spectral analysis indicated that cytochrome b was present in the red band: the reduced minus oxidized difference spectra revealed absorption maxima at 562,527, and 431 nm at room temperature and 562, 530, and 422 nm at 77K. In addition, a 28-kDa protein was identified in this fraction which retained heme-associated peroxidase activity even after denaturation on SDS-polyacrylamide gels. The amino acid composition of this protein showed a strong similarity to cytochrome c1 of both bovine and yeast.
Details
- Language :
- English
- ISSN :
- 0014-4894
- Volume :
- 68
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 2542079
- Full Text :
- https://doi.org/10.1016/0014-4894(89)90129-x