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Sodium/proton antiport is required for growth of Escherichia coli at alkaline pH.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1989 May 19; Vol. 981 (1), pp. 21-6. - Publication Year :
- 1989
-
Abstract
- Evidence is presented indicating that Escherichia coli requires the Na+/H+ antiporter and external sodium (or lithium) ion to grow at high pH. Cells were grown in plastic tubes containing medium with a very low Na+ content (5-15 microM). Normal cells grew at pH 7 or 8 with or without added Na+, but at pH 8.5 external Na was required for growth. A mutant with low antiporter activity failed to grow at pH 8.5 with or without Na+. On the other hand, another mutant with elevated antiporter activity grew at a higher pH than normal (pH 9) in the presence of added Na+ or Li+. Amiloride, an inhibitor of the antiporter, prevented cells from growing at pH 8.5 (plus Na+), although it had no effect on growth in media of lower pH values.
- Subjects :
- Amiloride pharmacology
Culture Media
Escherichia coli genetics
Escherichia coli metabolism
Growth Inhibitors pharmacology
Hydrogen-Ion Concentration
Lithium pharmacology
Mutation
Sodium pharmacology
Sodium-Hydrogen Exchangers
Carrier Proteins physiology
Escherichia coli growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 981
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 2541789
- Full Text :
- https://doi.org/10.1016/0005-2736(89)90077-1