Back to Search
Start Over
Myeloma cells act as tolerogenic antigen-presenting cells and induce regulatory T cells in vitro.
- Source :
-
European journal of haematology [Eur J Haematol] 2015 Jul; Vol. 95 (1), pp. 65-74. Date of Electronic Publication: 2015 Feb 23. - Publication Year :
- 2015
-
Abstract
- Regulatory T cells (Tregs) are essential for maintenance of self-tolerance; however, tumor cells can exploit the tolerance to escape the immune system. We investigated the Tregs frequency in patients with multiple myeloma (MM) and in those with monoclonal gammopathy of undetermined significance (MGUS), and found that CD4(+) FoxP3(+) and CD8(+) FoxP3(+) Tregs were significantly increased in patients with MM and correlated with the active phase. Both Tregs subsets were expanded in cocultures of CD3(+) lymphocytes and fresh CD138(+) MM plasma cells or RPMI8226 and U266 cell lines and functioned as natural (n) and inducible (i) Tregs insofar as they inhibited the proliferation of stimulated CD3 lymphocytes via contact-dependent and contact-independent pathways. Induction of Tregs by MM plasma cells required a contact-dependent pathway, implying antigen recognition by T cells. MM plasma cells acted as immature and tolerogenic antigen-presenting cells (APCs), in that they displayed low CD80/CD86 expression associated with a phagocytic activity. By acting as immature APCs, MM plasma cells plausibly expand (n)Tregs and (i)Tregs both through conversion of CD3(+) FoxP3(-) into CD3(+) FoxP3(+) T cells and proliferation of CD3(+) FoxP3(+) T cells, which may suppress the anti-MM immune response.<br /> (© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Antigen-Presenting Cells pathology
CD3 Complex genetics
CD3 Complex immunology
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes pathology
Cell Communication
Cell Proliferation
Coculture Techniques
Forkhead Transcription Factors genetics
Forkhead Transcription Factors immunology
Humans
Immune Tolerance
Immunophenotyping
Lymphocyte Activation
Monoclonal Gammopathy of Undetermined Significance genetics
Monoclonal Gammopathy of Undetermined Significance pathology
Multiple Myeloma genetics
Multiple Myeloma pathology
Plasma Cells pathology
Signal Transduction
Syndecan-1 genetics
Syndecan-1 immunology
T-Lymphocytes, Regulatory pathology
Antigen-Presenting Cells immunology
Gene Expression Regulation, Neoplastic
Monoclonal Gammopathy of Undetermined Significance immunology
Multiple Myeloma immunology
Plasma Cells immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0609
- Volume :
- 95
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of haematology
- Publication Type :
- Academic Journal
- Accession number :
- 25409753
- Full Text :
- https://doi.org/10.1111/ejh.12481