Comparison of conventional and sustained delivery of antioxidants to suppress the caov-3 cell lin in ovarian cancer.

The objective of this study was to investigate the synergistic effect of Thymoquinone (TQ) and Epigallocatechin-3-gallate (EGCG) using Caov-3 cell line as a model. A total of 144 wells were plated with 10^5 Caov-3 ovarian cancer cells. The wells were divided into groups of 72 wells for conventional and sustained delivery, respectively. Each group was subdivided into 4 groups of 6 wells. Group 1 served as control and groups 2, 3, and 4 were treated with TQ (16 µM), EGCG (3 µg/ml), and TQ plus EGCG, respectively. Biomarkers evaluations were performed following standard lab techniques. The results of the study revealed: conventional delivery of TQ, EGCG, and the combination did not induce intracellular oxidative stress (glutathione levels) at 24, 48, and 72 hours; however, sustained delivery of all agents showed significant difference from the control at 72 hours; and there was no significant difference at 24 and 48 hours. Overall conclusion: the route of potent agents to manipulate ovarian cancer cells is highly dependent on the route of administration.