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Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1220, pp. 339-45. - Publication Year :
- 2015
-
Abstract
- The production of nitric oxide in mast cells has been difficult to measure due to the low amounts made by mast cells, as well as limitations in the specificity and sensitivity of the assays available. We present here a sensitive and specific 96-well plate-based method to directly measure NO using the cell-permeable fluorescent compound DAF-FM diacetate.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1220
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 25388261
- Full Text :
- https://doi.org/10.1007/978-1-4939-1568-2_21