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Isolation of a transposable element from Neurospora crassa.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1989 Mar; Vol. 86 (6), pp. 1929-33. - Publication Year :
- 1989
-
Abstract
- A Neurospora crassa strain from Adiopodoumé, Ivory Coast, contains multiple copies of a transposable element, Tad. The element was detected as a 7-kilobase insertion in two independently isolated spontaneous forward mutants of the am (glutamate dehydrogenase) gene. Laboratory strains do not contain Tad. All progeny from crosses of the Adiopodoumé strain to laboratory strains contain multiple copies. When the element was inserted in am, target sequences of 14 and 17 base pairs were duplicated in the two cases analyzed. One mutation, caused by the insertion of Tad at the beginning of the am coding sequence, is genetically stable. The other mutation, caused by insertion upstream of the transcriptional start site, has a reversion frequency of 2.5 x 10(-3). Precise excisions of Tad have not been found.
- Subjects :
- Base Sequence
Cloning, Molecular
DNA Probes
DNA, Fungal genetics
DNA, Mitochondrial genetics
Deoxyribonuclease BamHI
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
Glutamate Dehydrogenase genetics
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
DNA Transposable Elements
Genes, Fungal
Neurospora genetics
Neurospora crassa genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 86
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 2538822
- Full Text :
- https://doi.org/10.1073/pnas.86.6.1929