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Drought stress tolerance in grapevine involves activation of polyamine oxidation contributing to improved immune response and low susceptibility to Botrytis cinerea.
- Source :
-
Journal of experimental botany [J Exp Bot] 2015 Feb; Vol. 66 (3), pp. 775-87. Date of Electronic Publication: 2014 Nov 10. - Publication Year :
- 2015
-
Abstract
- Environmental factors including drought stress may modulate plant immune responses and resistance to pathogens. However, the relationship between mechanisms of drought tolerance and resistance to pathogens remained unknown. In this study, the effects of drought stress on polyamine (PA) homeostasis and immune responses were investigated in two grapevine genotypes differing in their drought tolerance; Chardonnay (CHR), as sensitive and Meski (MSK), as tolerant. Under drought conditions, MSK plants showed the lowest leaf water loss and reduction of photosynthetic efficiency, and expressed a lower level of NCED2, a gene involved in abscisic acid biosynthesis, compared with CHR plants. The improved drought tolerance in MSK was also coincident with the highest change in free PAs and up-regulation of the genes encoding arginine decarboxylase (ADC), copper amine-oxidase (CuAO), and PA-oxidases (PAO) and their corresponding enzyme activities. MSK plants also accumulated the highest level of amino acids, including Arg, Glu, Gln, Pro, and GABA, emphasizing the participation of PA-related amino acid homeostasis in drought tolerance. Importantly, drought-tolerant plants also exhibited enhanced phytoalexin accumulation and up-regulation of PR genes, especially PR-2 and Chit4c, compared with the sensitive plants. This is consistent with a lower susceptibility of MSK than CHR to Botrytis cinerea. Data suggest a possible connection between water stress tolerance and immune response in grapevine. Pharmacological experiments revealed that under drought conditions CuAO and PAO pathways were involved in the regulation of photosynthetic efficiency, and also of immune response and resistance of grapevine to a subsequent pathogen attack. These results open new views to improve our understanding of crosstalk between drought tolerance mechanisms and immune response.<br /> (© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Amine Oxidase (Copper-Containing) metabolism
Homeostasis
Oxidation-Reduction
Oxidoreductases Acting on CH-NH Group Donors metabolism
Photosystem II Protein Complex metabolism
Plant Leaves enzymology
Plant Leaves genetics
Plant Leaves immunology
Plant Leaves physiology
Stress, Physiological
Vitis genetics
Vitis immunology
Polyamine Oxidase
Botrytis physiology
Droughts
Plant Immunity
Polyamines metabolism
Vitis microbiology
Vitis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2431
- Volume :
- 66
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 25385768
- Full Text :
- https://doi.org/10.1093/jxb/eru436