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Transactivation of large ribozymes.

Authors :
Martin MB
Leeper TL
Schmidt FJ
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1240, pp. 57-62.
Publication Year :
2015

Abstract

The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure-function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.

Details

Language :
English
ISSN :
1940-6029
Volume :
1240
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
25352137
Full Text :
https://doi.org/10.1007/978-1-4939-1896-6_5