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Transactivation of large ribozymes.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1240, pp. 57-62. - Publication Year :
- 2015
-
Abstract
- The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure-function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.
- Subjects :
- Bacillus subtilis enzymology
Base Sequence
Molecular Sequence Data
Mutation genetics
RNA, Catalytic chemistry
RNA, Catalytic metabolism
Ribonuclease P chemistry
Ribonuclease P genetics
Ribonuclease P metabolism
Molecular Biology methods
RNA, Catalytic genetics
Transcriptional Activation genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1240
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 25352137
- Full Text :
- https://doi.org/10.1007/978-1-4939-1896-6_5