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Identification of antisense RNA stem-loops that inhibit RNA-protein interactions using a bacterial reporter system.

Authors :
Harada K
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1240, pp. 49-56.
Publication Year :
2015

Abstract

RNA-protein interactions play important roles in gene regulation, functional RNA-protein complexes such as the ribosome, and in viral replication. Molecules that regulate specific RNA-protein interactions may be used to dissect biological processes, and to establish the validity of targeting an RNA-protein interaction. There are many examples of biological regulation by antisense RNA stem-loops that form loop-loop and loop-linear RNA-RNA interactions. Here, a bacterial reporter system for the identification of RNA stem-loops that inhibit the formation of RNA-protein complexes through RNA-RNA interactions is described.

Details

Language :
English
ISSN :
1940-6029
Volume :
1240
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
25352136
Full Text :
https://doi.org/10.1007/978-1-4939-1896-6_4