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[Detection of conservative and variable epitopes of the pandemic influenza virus A(H1N1)pdm09 hemagglutinin using monoclonal antibodies].
- Source :
-
Voprosy virusologii [Vopr Virusol] 2014 May-Jun; Vol. 59 (3), pp. 34-40. - Publication Year :
- 2014
-
Abstract
- The goal of this work was to analyze the antigenic structure of the hemagglutinin (HA) of the pandemic influenza virus A(H1N1)pdm09 using monoclonal antibodies (MAbs) and to develop a sandwich ELISA for identification of pandemic strains. Competitive ELISA demonstrated that 6 MAbs against HA of the pandemic influenza A/ IIV-Moscow/01/2009 (H1N1)pdm09 virus identified six epitopes. Binding of MAbs with 22 strains circulating in Russian Federation during 2009-2012 was analyzed in the hemagglutination-inhibition test (HI). The MAbs differed considerably in their ability to decrease the HI activity of these strains. MAb 5F7 identified all examined strains; MAbs 3A3 and 10G2 reacted with the majority of them. A highly sensitive sandwich ELISA was constructed based on these three MAbs that can differentiate the pandemic influenza strains from the seasonal influenza virus. The constancy of the HA epitope that reacts with MAb 5F7 provides its use for identification of the pandemic influenza strains in HI test. MAbs 3D9, 6A3 and 1E7 are directed against the variable HA epitopes, being sensitive to several amino acid changes in Sa, Sb, and Ca2 antigenic sites and in receptor binding site. These MAbs can be used to detect differences in HA structure and to study the antigenic drift of the pandemic influenza virus A(H1N1)pdm09.
- Subjects :
- Antibodies, Monoclonal chemistry
Antibodies, Monoclonal immunology
Antibodies, Viral immunology
Antibody Specificity
Antigens, Viral genetics
Antigens, Viral immunology
Enzyme-Linked Immunosorbent Assay
Epitopes genetics
Epitopes immunology
Genetic Drift
Hemagglutinins genetics
Hemagglutinins immunology
Humans
Influenza A Virus, H1N1 Subtype chemistry
Influenza A Virus, H1N1 Subtype genetics
Influenza, Human immunology
Influenza, Human virology
Moscow epidemiology
Antibodies, Viral chemistry
Antigens, Viral chemistry
Epitopes chemistry
Hemagglutinins chemistry
Influenza A Virus, H1N1 Subtype immunology
Influenza, Human epidemiology
Pandemics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0507-4088
- Volume :
- 59
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Voprosy virusologii
- Publication Type :
- Academic Journal
- Accession number :
- 25335417