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CD271 mediates stem cells to early progeny transition in human epidermis.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2015 Mar; Vol. 135 (3), pp. 786-795. Date of Electronic Publication: 2014 Oct 20. - Publication Year :
- 2015
-
Abstract
- CD271 is the low-affinity neurotrophin (p75NTR) receptor that belongs to the tumor necrosis factor receptor superfamily. Because in human epidermis, CD271 is predominantly expressed in transit-amplifying (TA) cells, we evaluated the role of this receptor in keratinocyte differentiation and in the transition from keratinocyte stem cells (KSCs) to progeny. Calcium induced an upregulation of CD271 in subconfluent keratinocytes, which was prevented by CD271 small interfering RNA. Furthermore, CD271 overexpression provoked the switch of KSCs to TA cells, whereas silencing CD271 induced TA cells to revert to a KSC phenotype, as shown by the expression of β1-integrin and by the increased clonogenic ability. CD271(+) keratinocytes sorted from freshly isolated TA cells expressed more survivin and keratin 15 (K15) compared with CD271(-) cells and displayed a higher proliferative capacity. Early differentiation markers and K15 were more expressed in the skin equivalent generated from CD271(+) TA than from those derived from CD271(-) TA cells. By contrast, late differentiation markers were more expressed in skin equivalents from CD271(-) than in reconstructs from CD271(+) TA cells. Finally, skin equivalents originated from CD271(-) TA cells displayed a psoriatic phenotype. These results indicate that CD271 is critical for keratinocyte differentiation and regulates the transition from KSCs to TA cells.
- Subjects :
- Calcium pharmacology
Cell Differentiation drug effects
Cells, Cultured
Epidermis drug effects
Epidermis metabolism
Humans
In Vitro Techniques
Inhibitor of Apoptosis Proteins metabolism
Keratin-15 metabolism
Keratinocytes drug effects
Keratinocytes metabolism
Nerve Tissue Proteins deficiency
Nerve Tissue Proteins genetics
Phenotype
Psoriasis pathology
RNA, Small Interfering pharmacology
Receptors, Nerve Growth Factor deficiency
Receptors, Nerve Growth Factor genetics
Stem Cells drug effects
Stem Cells metabolism
Survivin
Cell Differentiation physiology
Epidermal Cells
Keratinocytes cytology
Nerve Tissue Proteins metabolism
Receptors, Nerve Growth Factor metabolism
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 135
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 25330297
- Full Text :
- https://doi.org/10.1038/jid.2014.454