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Redistribution of carbon flux toward 2,3-butanediol production in Klebsiella pneumoniae by metabolic engineering.
- Source :
-
PloS one [PLoS One] 2014 Oct 20; Vol. 9 (10), pp. e105322. Date of Electronic Publication: 2014 Oct 20 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Klebsiella pneumoniae KCTC2242 has high potential in the production of a high-value chemical, 2,3-butanediol (2,3-BDO). However, accumulation of metabolites such as lactate during cell growth prevent large-scale production of 2,3-BDO. Consequently, we engineered K. pneumoniae to redistribute its carbon flux toward 2,3-BDO production. The ldhA gene deletion and gene overexpression (budA and budB) were conducted to block a pathway that competitively consumes reduced nicotinamide adenine dinucleotide and to redirect carbon flux toward 2,3-BDO biosynthesis, respectively. These steps allowed efficient glucose conversion to 2,3-BDO under slightly acidic conditions (pH 5.5). The engineered strain SGSB105 showed a 40% increase in 2,3-BDO production from glucose compared with that of the host strain, SGSB100. Genes closely related to 2,3-BDO biosynthesis were observed at the gene transcription level by cultivating the SGSB100, SGSB103, SGSB104, and SGSB105 strains under identical growth conditions. Transcription levels for budA, budB, and budC increased approximately 10% during the log phase of cell growth relative to that of SGSB100. Transcription levels of 2,3-BDO genes in SGSB105 remained high during the log and stationary phases. Thus, the carbon flux was redirected toward 2,3-BDO production. Data on batch culture and gene transcription provide insight into improving the metabolic network for 2,3-BDO biosynthesis for industrial applications.
- Subjects :
- Batch Cell Culture Techniques
Fermentation
Klebsiella pneumoniae enzymology
Klebsiella pneumoniae growth & development
L-Lactate Dehydrogenase genetics
Mutation
Transcription, Genetic genetics
Butylene Glycols metabolism
Carbon metabolism
Klebsiella pneumoniae genetics
Klebsiella pneumoniae metabolism
Metabolic Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25329548
- Full Text :
- https://doi.org/10.1371/journal.pone.0105322