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Novel rolling circle amplification and DNA origami-based DNA belt-involved signal amplification assay for highly sensitive detection of prostate-specific antigen (PSA).
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2014 Nov 26; Vol. 6 (22), pp. 20372-7. Date of Electronic Publication: 2014 Oct 28. - Publication Year :
- 2014
-
Abstract
- Prostate-specific antigen (PSA) is one of the most important biomarkers for the early diagnosis and prognosis of prostate cancer. Although many efforts have been made to achieve significant progress for the detection of PSA, challenges including relative low sensitivity, complicated operation, sophisticated instruments, and high cost remain unsolved. Here, we have developed a strategy combining rolling circle amplification (RCA)-based DNA belts and magnetic bead-based enzyme-linked immunosorbent assay (ELISA) for the highly sensitive and specific detection of PSA. At first, a 96-base circular DNA template was designed and prepared for the following RCA. Single stranded DNA (ssDNA) products from RCA were used as scaffold strand for DNA origami, which was hybridized with three staple strands of DNA. The resulting DNA belts were conjugated with multiple enzymes for signal amplification and then employed to magnetic bead based ELISA for PSA detection. Through our strategy, as low as 50 aM of PSA can be detected with excellent specificity.
- Subjects :
- DNA metabolism
DNA, Single-Stranded chemistry
DNA, Single-Stranded metabolism
Humans
Male
Nanostructures chemistry
Nucleic Acid Amplification Techniques
Prostate-Specific Antigen genetics
Prostatic Neoplasms diagnosis
Sensitivity and Specificity
DNA chemistry
Enzyme-Linked Immunosorbent Assay
Prostate-Specific Antigen analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 6
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 25323204
- Full Text :
- https://doi.org/10.1021/am505913d